Table 1. HPLC analyses of UGT activities of protein extracts from Pichia pastoris and Arabidopsis plants.
Pichia pastoris
|
Arabidopsis thaliana
|
|||||
---|---|---|---|---|---|---|
CK | UGT | WT | UGT-1 | UGT-2 | UGT-3 | |
2,4,5-TCP | 0 | 230.55±15.09 | 7.30±0.60 | 10.95±0.83** | 10.50±0.82** | 9.06±0.74* |
2,6-DMP | 0 | 96.24±8.24 | 1.17±0.14 | 1.61±0.12** | 1.58±0.13** | 1.49±0.11* |
phenol | 0 | 104.29±8.17 | 4.28±0.34 | 8.84±0.72** | 7.82±0.62** | 6.53±0.57** |
hydroquinone | 0 | 71.16±8.67 | 7.06±0.63 | 11.59±0.94** | 10.49±0.85** | 9.14±0.76* |
catechol | 6.52±0.53 | 130.36±14.39 | 5.65±0.54 | 9.33±0.86** | 7.90±0.58** | 6.81±0.57 |
3-MC | 0 | 23.67±2.28 | 0.85±0.06 | 0.92±0.06 | 1.02±0.08* | 0.98±0.07 |
3,4-DCA | 0 | 68.64±4.06 | 4.37±0.29 | 5.45±0.45* | 6.06±0.40** | 5.04±0.38 |
Data represent mean ± SD (n = 3). Statistical analyses of differences in VT Arabidopsis plants with respect to WT Arabidopsis plants and P. pastoris expressing VvUGT72B1 with respect to P. pastoris transformed with pPIC9K are performed using Dunnett’s two-tailed t-test. Significant difference is denoted with one (P<0.05) or (P<0.01) two asterisks.