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. 2013 Nov 14;8(11):e80730. doi: 10.1371/journal.pone.0080730

Figure 5. Decreased sensitivity to apoptosis-related proteins in late passage -/- MEF cells.

Figure 5

(A) Clonogenicity of +/+ and -/- MEFs at P30. The asterisks represent significant differences (*P <0.05) between +/+ and -/- MEFs. (B) Clonogenicity of MEF+/+ 3 and MEF-/- 5 cells after treatment with 20 µM DMBA for 24 h (P31). Bar graphs represent the means, and error bars the standard deviation from at least 3 independent experiments; (*P <0.05). (C, D) Western blots for Caspase 3, PARP, Survivin, Mcl1, p53, p21 and GAPDH expression in MEF+/+ 3 and -/- 5 cells before and 6 and 24 h after 20 µM DMBA treatment at P31. Note that although the +/+ and -/- MEFs show similar expression patterns for cleaved Caspase 3 and cleaved PARP after DMBA treatment, the MEF-/-5 cells likely survive DMBA treatment as shown in (B) because Survivin and Mcl1 are up-regulated in these cells by 24 h DMBA treatment, in contrast to the loss of these proteins at 24 h after DMBA in the MEF+/+3 cells.