Figure 2. Responses of human α4β2, α3β4 and α7-expressing cell lines to various concentrations of nicotinic agonists.

Responses were measured using the FLEXStation with an indicator sensitive to changes in membrane potential. Results are expressed as a percent of maximal fluorescence. Each data point is an average of the peak fluorescence of 4–8 individual dose-response curves. Nicotine and ACh are full agonists on α4β2, whereas varenicline, cytisine and sazetidine-A are partial agonists. All of the tested compounds are full agonists on α3β4 and α7 AChRs.