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. 2013 Nov 14;8(11):e81361. doi: 10.1371/journal.pone.0081361

Figure 4. Olfactory responses to ethyl acetate by TK-7 and other dsRNA treatment using single sensillum recording.

Figure 4

(A) Saline injection caused no significant changes after injection (N=5, p>0.5). (B) Application of TK-7 induced a substantial decrease in the spike activities of olfactory receptor neurons (N=4, p<0.001). (C) Control non-specific dsRNA did not alter olfactory responses (N=4, p>0.2). (D) Octopamine receptor (PaOA1) dsRNA treatment resulted in significant increase in spike numbers (N=5, p<0.01). (E) Tachykinin (PaTK) dsRNA injection exhibited no significant changes in olfactory responses at phase II (4~8th pulses) (N=4, p>0.9) but significant increase in phase III (after 9th pulse) (N=4, p<0.02). (F) Tachykinin receptor (PaTKR) dsRNA injection did not show significant changes (N=5, p>0.7). (G) Representative olfactory response traces by SSR upon TK-7 injection. Significant decrease of spike trains was shown, indicative of decrease of olfactory sensitivity. One-way ANOVA test followed by Bonferroni correction for multiple comparisons was employed for statistical analysis.