Table 3. qPCR analysis data of the relative estimation of BoR300 repeats using a reference standard curve.
| Sample concentration (pg)a | Calibration curve | Repeat copy numberb | Copy number per haploid genomec | |
| BoR300 | 10 | y = −3.52×log(x) +35.197 | 75,610±2,893d | 2,661 |
a
Initial template concentration of the B. oleae genomic DNA (pg) used at the qPCR reactions.
b
Mean copy number of BoR300 repeats which was estimated for the initial template concentration of the genomic DNA (a) based on the standard curve.
c
B. oleae haploid genome size: 0.352 pg.
d
Standard Error (SE) for the triplicate measurements (n = 3).