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. 2013 Nov 14;8(11):e79405. doi: 10.1371/journal.pone.0079405

Figure 4. The effect of Mpc overexpression on respiratory growth and oxidative stress tolerance.

Figure 4

Individual Mpc proteins were overexpressed as dsRed fusions in yeast wild type cells. A. All three Mpc-dsRed fusion proteins localize exclusively to the mitochondria. Yeast cells were cotransformed with the respective Mpc overexpressing constructs and the mitochondrial GFP expressing pVT100 plasmid. Representative pictures are shown for GFP, dsRed and the overlay obtained by confocal fluorescence microscopy. B. The overexpression of Mpc1 or Mpc2 inhibits respiratory growth. The growth of yeast wild type cells overexpressing the indicated Mpc proteins was assayed on glucose (SD) or glycerol (SGlyc) containing minimal medium and compared to the respective empty vector control. C. Overexpression of Mpc3 leads to oxidative stress tolerance. The growth of yeast strains described in (B) was continuously monitored in normal medium (SD) and in the presence of 2 mM hydrogen peroxide or 25 µM menadione. Data presented are mean values from three biological replicates. The standard deviation is 1–3% for SD, 1–2% for hydrogen peroxide and 2–10% for menadione growth.