Table 1. Motility rates for Myo2p purified with and without Rng3p function.

Shift time (hours)*		25°C (µm/sec)		37°C (µm/sec)
16	wild-type	0.42±0.12		0.40±0.14
	rng3-65	0.30±0.10		0.03±0.02
22	wild-type	0.46±0.07		0.42±0.06
	rng3-65	0.34±0.05		0.32±0.06

All values are the mean filament velocity ± SD.

^*Shift time is the amount of time following the induction of Myo2p over-expression at which cells were shifted to 37°C to attenuate Rng3p function (in the rng3-65 strain). Values for wild-type cells are provided as a control. 25°C values represent internal controls that were not shifted to 37°C. At the 16 hour shift Rng3p function is compromised prior to Myo2p over-expression; at the 22 hour shift Rng3p function is compromised following over-expression of Myo2p (see Figure 2A).