Figure 3.

Dhh^Cre;Gpr126^fl/fl SCs fail to generate myelin. A, B, Sciatic nerves (arrows) from P21 littermates. A, Control (Gpr126^fl/fl shown) nerves are white and opaque. B, Dhh^Cre;Gpr126^fl/fl nerves are translucent. C–H, Representative toluidine blue-stained semithin sections of sciatic nerves from sibling control and Dhh^Cre;Gpr126^fl/fl mice. C, E, G, Many myelinated axons are observed in control sciatic nerves at P10 (C, Gpr126^fl/fl shown), P21 (E, Dhh^Cre;Gpr126^fl/+ shown), and P42 (G, Dhh^Cre;Gpr126^fl/+ shown). We did not observe differences between any control genotype at any stage examined. Myelinated axons are not observed in Dhh^Cre;Gpr126^fl/fl nerves at P10 (D), P21 (F), or P42 (H). Scale bar: (in C) C–H, 10 μm. C–H, Black arrows denote myelin sheaths, and white arrows denote SC nuclei.