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. 2013 Nov 13;33(46):18015–18021. doi: 10.1523/JNEUROSCI.2290-13.2013

Figure 3.

Figure 3.

Hap1 controls Tsc1 abundance and S6 phosphorylation. A, Lysates of HEK293T cells transfected with FLAG-Tsc1 alone or FLAG-Tsc1 together with FLAG-Tsc2 or Myc-Hap1B were subjected to immunoblotting. Coexpression of Myc-Hap1B or FLAG-Tsc2 promoted FLAG-Tsc1 abundance. Arrow indicates location of Tsc1. B, Lysates of DIV5 hippocampal neurons infected on DIV0 2 h after plating with Hap1 RNAi or control lentivirus cultured under basal conditions were subjected to immunoblotting. Hap1 knockdown triggered downregulation of Tsc1 and Tsc2. The i.1, i.3 nomenclature refers to individual shRNAs. C, Lysates of DIV5 hippocampal neurons infected on DIV0 2 h after plating with RNAi cultured under basal conditions were subjected to immunoblotting. Hap1 knockdown triggered downregulation of Tsc1 and Tsc2 and stimulated phosphorylation of the mTORC1 effector S6.