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. 2013 Nov 1;27(21):2345–2355. doi: 10.1101/gad.223800.113

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Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 5. — JNK deficiency causes increased TSH expression. (A) The blood concentration of TSH, T3, T4, and free T4 in ND-fed and HFD-fed (4 wk) P^WT and P^ΔJ1,J2 mice following overnight starvation was measured by ELISA (mean ± SEM; n = 8∼12). (*) P < 0.05. (B) Sections of the pituitary gland were stained with an antibody to TSHβ. DNA was stained with DAPI. The anterior lobe (AL) and posterior lobe (PL) of the pituitary gland are indicated. (C) Total RNA was isolated from primary cultures of pituitary cells isolated from control and JNK-deficient mice. Gene expression was measured by quantitative RT–PCR analysis of mRNA (mean ± SEM; n = 6∼8). (*) P < 0.05; (**) P < 0.01.