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. 2013 Nov 1;27(21):2356–2366. doi: 10.1101/gad.227512.113

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© 2013 Chuprin et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 4. — ERVWE1-induced and MV-induced cell fusion induces the p53-dependent senescence phenotype in cancer cells. (A,B) SA-β-gal activity was examined in a syncytial (Syn) and a mononuclear (Mono) population of A549 cells expressing sh-LacZ or sh-p53 and transduced with ERVWE1 or GFP as a control. Bar, 100 μm. Arrowheads indicate syncytia. (C) Expression of p53 protein in A549 cells expressing sh-LacZ or sh-p53 and transduced with ERVWE1 (ERV) or control vector (Ctrl). (D,E) BrdU incorporation was assayed in a syncytial and a mononuclear population of A549 cells expressing sh-LacZ or sh-p53 and transduced with ERVWE1. Bar, 50 μm. Arrowheads indicate syncytia. (F) Expression of CCL5 and IL-8 in A549 cells expressing sh-LacZ or sh-p53 and transduced with ERVWE1 (ERV) or control vector (Ctrl) was measured by quantitative RT–PCR. (G) Soft agar colony formation assay of A549 cells expressing sh-LacZ or sh-p53 and transduced with ERVWE1 or control vector. SA-β-gal activity (H) and BrdU incorporation (I) were examined in a syncytial (Syn) and a mononuclear (Mono) population of A549 cells expressing sh-LacZ or sh-p53 and infected with MV. Values are means + SEM; (*) P < 0.05; (**) P < 0.01.