Fig. 5.
Genetic manipulation places Dim7 upstream of the Elmo–Mbc complex. (A,B,D–G) MHC staining of stage 16–17 embryos. (A–C) Excess Dim7 expression in myofibers results in the separation of muscles from smaller MASs (A). This muscle detachment phenotype is suppressed by removal of one copy of mbcD11.2 (B,C). (D–H) Expression of Elmo–Mbc→Rac signaling components rescues the muscle defects in msk mutants. The severe attachment defects observed in msk4/msk4 embryos (D) are partially restored upon muscle-specific expression of Elmo (E). Improved restoration of muscle attachment is observed by introduction of the Elmo–Mbc complex (F) or RacV12 (G). (H) Bar graph quantifying the rescue of muscle attachment defects in msk4/4 mutant embryos upon muscle-specific expression of Elmo, the Elmo–Mbc complex, the ElmoEDE variant or RacV12.