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. 2013 Dec;94(6):1309–1315. doi: 10.1189/jlb.0113043

Figure 2. AR stimulation augments the IL-10-induced expression of TIMP-1 and arginase-1 mRNA.

Figure 2.

(A) TIMP-1 mRNA expression in RAW 264.7 cells treated with 10 ng/ml IL-10 or 100 μM adenosine and 10 ng/ml IL-10 for 6 h. ###P < 0.001 versus IL-10 (n=6). (B) Arginase-1 (Arg-1) mRNA expression of RAW 264.7 cells treated with 1 μM NECA or 10 ng/ml IL-10 or 1 μM NECA and 10 ng/ml IL-10 for 6 h. *P < 0.05, versus vehicle; ##p < 0.01 versus IL-10 (n=6). (C) Arginase-1 mRNA expression of BMDMs, isolated from WT, A2AAR, or A2BAR mice, which were treated with 1 μM NECA and/or 10 ng/ml IL-10 for 6 h. *P < 0.05, **P < 0.01, ***P < 0.001 (n=3). (D) SOCS-3 mRNA expression of WT, A2AAR, or A2BAR KO BMDMs treated with 10 ng/ml IL-10 or 1 μM NECA and 10 ng/ml IL-10 for 6 h. **P < 0.01, ***P < 0.001 versus vehicle (n=6). Results are the summary of two independent experiments. (E) BCL-3 mRNA expression of RAW 264.7 cells treated with IL-10 or 1 μM NECA and 10 ng/ml IL-10 for 6 h. **P < 0.01 versus vehicle (n=6). All results (mean±sem) are representative of at least three independent experiments.