Skip to main content
. 2013 Dec;94(6):1309–1315. doi: 10.1189/jlb.0113043

Figure 3. AR stimulation augments IL-10-induced STAT3 phosphorylation.

Figure 3.

(A) Phosphorylated STAT3 (pSTAT3) and actin protein expression in RAW 264.7 cells treated with 10 ng/ml IL-10 or 1 μM NECA and 10 ng/ml IL-10 for 60 min. (B) STAT3 and actin protein expression in RAW 264.7 cells transfected with NT or STAT3-specific siRNA. (C) TIMP-1 production by RAW 264.7 cells transfected with NT or STAT3-specific siRNA and treated with 10 ng/ml IL-10 or 1 μM NECA and 10 ng/ml IL-10 for 6 h. *P < 0.05, ***P < 0.001 (mean±sem; n=4). (D) TIMP-1 production by RAW 264.7 cells treated with 10 μM AG490, 10 μM ZM39923, or 1 μM cucurbitacin, 30 min before treatment with 1 μM NECA and 10 ng/ml IL-10 for 24 h. ***P < 0.001 versus vehicle (mean±sem; n=5). (E) TIMP-1 production by RAW 264.7 cells treated with 100 nM SB203580 or LY294002, 30 min before treatment with 1 μM NECA and 10 ng/ml IL-10 for 24 h. Data are shown as percent of control (NECA+IL-10). ***P < 0.001 versus vehicle (mean±sem; n=5). Results are representative of at least three independent experiments.