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. 2013 Dec;94(6):1309–1315. doi: 10.1189/jlb.0113043

Figure 4. AR stimulation inhibits IL-6-induced STAT3 phosphorylation.

Figure 4.

(A) pSTAT3 and actin protein expression in RAW 264.7 cells treated with 10 ng/ml IL-6 or 1 μM NECA and 10 ng/ml IL-6 for 1 h. (B) Densitometric analysis of Western blot results. ***P < 0.001 versus vehicle; ###P < 0.001 versus IL-6. SAA3 (C), TIMP-1 (D), or arginase-1 (E) mRNA expression in RAW 264.7 cells treated with 10 ng/ml IL-6 or 1 μM NECA and 10 ng/ml IL-6 for 6 h. **P < 0.01, ***P < 0.001 versus vehicle; ##P < 0.01, ###P < 0.001 versus IL-6 (mean±sem; n=6). Results are representative of at least three independent experiments. (F) Arginase-1 mRNA expression of BMDMs, isolated from WT, A2AAR, or A2BAR mice, which were treated with 1 μM NECA and/or 10 ng/ml IL-6 for 6 h. *P < 0.05, ***P < 0.001 (n=6). Results are a summary of two independent experiments.