Table 1.
List of currently studied mutations in Synechocystis sp. PCC 6803 glutaredoxin A.
| Mutation | Soluble | Insoluble | Crystallize | Δ #Neighbors | Pseudo ΔΔG kcal/mol|prediction | Final structure | |
|---|---|---|---|---|---|---|---|
| WT | ✓ | ✓ | – | – | Yes | ||
| A2Y | ✓ | – | +6 | −0.02 | n | ||
| A2I | ✓ | ✓ | +1 | −0.30 | n | ||
| A2T | ✓ | ✓ | +1 | −0.6 | sd | ||
| I9L | ✓ | ✓ | 0 | −0.59 | sd | ||
| T11Y | X | ✓ | – | +3 | +1.39 | s | |
| F17N | ✓ | – | −4 | −1.89 | d | ||
| M19A | ✓ | – | −2 | +0.91 | ss | ||
| A23N | ✓ | – | 0 | −3.62 | hd | ||
| L25S | X | ✓ | – | −3 | −4.56 | hd | |
| R27L | ✓ | ✓ | −2 | −0.08 | n | Yes | |
| R27V | ✓ | ✓ | −1 | −0.82 | sd | ||
| K28C | ✓ | ✓ | −1 | +0.56 | ss | ||
| G29C | ✓ | – | +2 | −0.44 | n | ||
| E34L | ✓ | – | 0 | +1.00 | ss | ||
| A43G | ✓ | – | −1 | −3.54 | hd | ||
| A43I | ✓ | ✓ | +2 | +0.27 | n | ||
| A43T | ✓ | ✓ | +3 | −2.29 | hd | ||
| A49H | ✓ | ✓ | +2 | −2.10 | hd | ||
| A49W | ✓ | ✓ | +4 | −2.32 | hd | ||
| A51E | ✓ | – | +4 | −2.84 | hd | ||
| R55A | ✓ | ✓ | −4 | +0.11 | n | ||
| G68Y | X | ✓ | – | +1 | +3.18 | hs | |
| G69F | ✓ | – | +3 | +1.83 | s | ||
| C70S | ✓ | – | 0 | −3.69 | hd | ||
| A75I | ✓ | ✓ | 0 | −1.19 | d | Yes | |
| A79S | ✓ | ✓ | 0 | −0.58 | ds | Yes | |
| P84R | ✓ | ✓ | +3 | +1.65 | s | Yes | |
Soluble = mutant protein could be purified from bacterial lysate.
Insoluble = no mutant protein could be purified from bacterial lysate.
Crystallize = were crystals of any quality grown?
Δ #Neighbors = residues which could cause potential collisions in this crystallographic setting.
Pseudo ΔΔG (kcal/mol) n, neutral; sd, slightly destabilizing; s, stabilizing; d, destabilizing; hd, highly destabilizing; ss, slightly stabilizing; hs, highly stabilizing.
Structure = Was a refined crystal structure produced?