Table 3.
ACC oxidase contains putative amino acid sequence motifs for post-translational modifications.
| N-glycosylation site |
| Consensus: Asn–X–Ser/Thr |
| ACCO: Asn99-Ile–Ser |
| O-glycosylation site |
| Consensus: Ser–X–X–Pro |
| ACCO: Ser101–Glu–Val–Pro |
| Protein consensus sequence motifs in ACCO |
| Membrane protein sorting signal: Tyr–X–X–Hydrophobic (Hyd is Leu, Ile, Met or Phe) |
| ACCO: Tyr277–Pro–Lys–Phe |
| ACCO: Tyr287–Met–Lys–Leu |
| ACCO: Tyr291–Ala–Gly–Leu |
| All in the C-terminal polypeptide proteolytic fragment Val266–Met309 |
| Phosphorylation site consensus sequences |
| Serine/threonine kinases |
| Cyclic-AMP-dependent kinase |
| Arg–X–X–Ser/Thr–(Hydrophobic) |
| ACCO: Arg–Ala–Ala–Thr20–Met |
| Ca-calmodulin-dependent protein kinase II |
| Arg–X–X–Ser/Thr–(Hydrophobic) |
| ACCO: Arg–Ala–Ala–Thr20–Met |
| Tyrosine protein kinase (Hydrophilic)1–3–X–Tyr–X–X– (Hydrophobic) |
| ACCO: Asp–Glu–Glu–Tyr110–Arg–Glu–Val (SH2 domain binding) |
| ACCO: Asp–Asp–Tyr287–Met–Lys–Leu (also membrane sorting signal) |
| ACCO: Asp–Trp–Glu–Ser–Tyr89–Phe–Phe–Leu |
| Protein–protein binding |
| SH2 domain BINDING (Src homology 2) |
| Consensus: Tyrp–X–X–Hydrophobic |
| ACCO: Tyr110–Arg–Glu–Val |
| ACCO: Tyr260–Pro–Ala–Pro |
| SH3 domain binding (Src homology 3) |
| Consensus: Pro–Pro–X–Pro |
| ACCO: Pro163–Pro–Cys–Pro |
| Coactivator motif (nuclear receptor transcriptional activation) |
| Consensus: Leu–X–X–Leu–Leu |
| ACCO: Leu–Leu–Asp–Leu–Leu |
| Transcriptional regulation motif |
| Leucine zipper |
| Consensus: Lys–X6–Leu–X6–Leu–X6–Leu–X6–Leu–X6–Leu |
| ACCO: Phe117–X6–Leu124–X6–Leu131–X6–Leu138– |
| Homology with cysteine proteases |
| LeCp: 197 SLVLYI E ACESGSI FE 212 |
| ++ + + ACE+ ++ |
| ACCO: 20 TMEMIKDACENWGFFE 35 |