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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Anal Biochem. 2013 Aug 29;443(1):10.1016/j.ab.2013.08.018. doi: 10.1016/j.ab.2013.08.018

Fig. 3.

Fig. 3

Time course of MazFSa endoribonuclease activity on an mRNA transcript. MazFSa(His)6 (1 nM) was incubated at room temperature with the single site substrate (from Fig. 2, Right; 25 nM). Activity is detected within 10 s and >50% processing is achieved by 5 min. To inhibit MazFSa activity, (His)6MazESa was incubated with MazFSa(His)6 for 15 min prior to reaction with the single site mRNA substrate for 60 seconds (+E lane). Products were separated by denaturing gel electrophoresis and visualized by ethidium bromide staining.