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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Mol Microbiol. 2010 Oct 18;78(6):10.1111/j.1365-2958.2010.07414.x. doi: 10.1111/j.1365-2958.2010.07414.x

Fig. 4.

Fig. 4

Oxidative stress and cell death in C. albicans. A. Effect of PMS on ROS generation in C. albicans cells determined by DCFH-DA staining. Cells were grown on YNBA with or without (control) 1 mM PMS, and with 10 mM H2O2 at 30°C. Fungal cells were harvested after 18 hours growth, washed and incubated with DCFH-DA for 30 minutes. Green fluorescence was evaluated by flow cytometry. B. C. albicans CAT1/CAT1 (WT CU2) or cat1/cat1 were grown on YNBA medium alone (control) and with PMS for 72 hours. Fungal survival determined by MB staining (n=3). Data are representative of at least two independent experiments ***P<0.01.