Fig. 6.
Reduction of PMS is required for its uptake and modification by C. albicans suspensions. C. albicans was grown on YNBA for 24 hours, and cells were then suspended in saline alone (control) or saline solution plus 100 mM ascorbic acid (AA), PMS (1mM) or PMS and AA (PMS+AA). A. Cells were analyzed by flow cytometry 4 hours post challenge to determine the levels of intracellular fluorescence. Mean fluorescence intensity (MFI) of each treatment is shown at the top of the FACS histogram. Data are representative of three independent experiments. B. C. albicans survival for each treatment as determined by CFU counts (n=3).