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. 2013 Nov 15;7:180. doi: 10.3389/fncir.2013.00180

FIGURE 2.

FIGURE 2

Trace eyeblink conditioning (EBC) is intact in cbln1-/- mice. (A) Diagram showing trace EBC. An unconditioned stimulus (US, 100 ms) was applied 500 ms after the end of the conditioned stimulus (CS, 350 ms) tone. Mice were trained for 7 days, one session per day. Associative learning was established when conditioned responses (CR), detected by electromyogram (EMG) were observed before unconditioned eyeblink responses (UR). (B) Intact trace EBC in cbln1-/- mice. No significant difference in CR% was observed between wild-type and cbln1-/- mice (p > 0.1, a two-way repeated measures ANOVA, n = 10 mice for each group). T1–T7, training sessions; Sp1 and Sp2, spontaneous eyeblink responses before training. The Y-axis indicates the percentage of trials showing CR. The dotted line corresponds to the percentage of CR-like activities on Sp2 to indicate the baselin e for learned responses. (C) Averaged EMG amplitude on the last day of training (T7). The EMG amplitudes were normalized to those during the initial 30 ms of CS; n = 10 for each group. No significant differences were observed between the two groups. (D) The effect of different time intervals on trace EBC. The interval between the end of the CS and the beginning of the US was shortened from 500 ms to 250 or 0 ms. The CR% on the last day of training (T7) is shown for each genotype. No significant CR% difference was observed for 500, 250, and 0 ms intervals between wild-type and cbln1-/- mice (p > 0.1, two-way repeated measures ANOVA followed by Bonferroni’s correction, n = 8 for each genotype).