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. 2013 Nov 15;7:180. doi: 10.3389/fncir.2013.00180

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Copyright © 2013 Emi, Kakegawa, Miura, Ito-Ishida, Kohda and Yuzaki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intact retention and expression of EBC in cbln1^-/- mice. (A) A diagram showing a sequence of experiments: cbln1^-/- mice were injected with Cbln1 as described in Figure 3 and subjected to delay eyeblink conditioning (EBC) from 2 (T1–T7) and at 40 days (T8, 30-days interval between T7 and T8) after the injection to examine the retention of learned responses. (B) Intact retention and expression of EBC in cbln1^-/- mice. During training (T1–T7) and retention (T8) sessions, no difference in CR% was observed between wild-type mice and cbln1^-/- mice injected with Cbln1 (p > 0.1, a two-way repeated measures ANOVA, n = 8 for each group). The dotted line corresponds to the percentage of CR-like activities on Sp2 to indicate the baseline for learned responses. (C) The level of CR% was similar between wild-type and cbln1^-/- mice injected with Cbln1 at T8 as well as at T7 (p > 0.1, by Mann-Whitney’s U test, n = 8 for each group).