Figure 7. A Pilot screen was performed using the NIH Clinical Collection Library.

MCF7-tet-on-shCARM1 cells were pre-treated with Dox for 7 days to knockdown endogenous CARM1. Pre-treated cells were batch-infected with 10% BacMam GFPPABP1 virus and 1% BacMam CARM1^WT virus, and then aliquoted 30 μl into 384-well plate. 0.3 μl 1 mM compounds were added with a robot to a final 10 μM concentration. 24 hours post-treatment, Me-GFP-PABP1 TR-FRET ratios were obtained and plotted as scatter plots. 10% BacMam CARM1^WT virus infection was used to provide the maximum activation range for Me-GFP-PABP1 TR-FRET ratio, and the Z’ factor was 0.76 for the pilot screen. Seven primary hits were denoted with circles.