Fig. 2. DDR1 over-expression enhances collagen fibril reorganization.

(A) Knockdown (KD), wild type (WT) or DDR1 over-expressing (OE) cells were cultured on FITC-labeled fibrillar collagen gels for 12 h (upper panel) or 24 h (lower panel) to evaluate morphologically, collagen remodeling. Bar = 20 µm; (B) Quantification of FITC-collagen fluorescence in fixed area regions of interest by image analysis using Image J. Mean fluorescent intensity was obtained from average fluorescence intensities normalized to data of same gels without cells. At least 4 representative images were analyzed for each condition. Data are mean±standard errors of fluorescence intensity in arbitrary units (AU) in sampling grids.