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. 2013 Sep 13;2(11):1148–1159. doi: 10.1242/bio.20135090

Fig. 5. DDR1 over-expression enhances total β1 integrin surface expression and activation.

Fig. 5.

(A) Cell surface activated β1 integrin on cells plated overnight on collagen. Immunostaining with 9EG7 antibody and analysis by flow cytometry of non-fixed, non-permeabilized cells. DDR1 over-expression increased total activated β1 integrin surface expression compared with wild type cells (P<0.05). (B) In cells plated on tissue culture plastic, staining of activated β1 integrin on cell surface (9EG7) is greater in DDR1 over-expressing cells than wild type cells (P<0.001). (C) Analysis of 9EG7 staining of cells in suspension. Activated β1 integrin on cell surface is greater in DDR1 over-expressing cells than wild type cells (**- P<0.01). Data are mean±S.E.M. (n = 3 independent experiments). (D) Same experimental approach as in panel C but cells were plated on non-tissue culture plastic, or non-tissue culture plastic coated with either 0.5 or 2 mg/ml collagen. (**- P<0.01). Data are mean±S.E.M. (n = 3 independent experiments).