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. 2013 Sep 17;2(11):1192–1202. doi: 10.1242/bio.20135603

Fig. 5. Expression of marshalin sub-isoforms during organ of Corti development.

Fig. 5.

RNA was isolated from single litters and collected at P0, P2, P4, P7, P10, P17, and adult (A). (A) RT-PCR using marshalin A7/B6 primers with expected bands: a. 245 bp; b. 212 bp; c. 191 bp; and d. 164 bp. The internal control gene cyclophilin has a band at 301 bp. Lane C1: no reverse transcriptase negative control. Lane C2: no single stranded cDNA template negative control. Lane S: positive controls using DNA plasmids encoding marshalin-Sa, marshalin-Sb, marshalin-Sc, and marshalin-Sd. (B) RT-PCR using marshalin A3/B4 with expected bands: marshalin-L (∼1800 bp) and marshalin-S (∼600 bp). The internal control gene G3PDH (glyceraldehyde 3-phosphate dehydrogenase) has a band around 900 bp. (C) Expression of marshalin proteins was analyzed by SDS-PAGE/Western blot. Marshalin-L and marshalin-S bands were identified in a P21 cochlea using anti-marshalin.