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. 2013 Oct 7;2(11):1229–1238. doi: 10.1242/bio.20136296

Fig. 3. Scl+19 activity marks haematopoietic cells and cardiac mesoderm in differentiating embryoid bodies.

Fig. 3.

(A) Fluorescent images of day 3–6 EBs from a representative Scl+19/Hsp/Venus clone. (B) Representative flow cytometry plots of Venus-YFP vs side scatter (SSC) of day 4 EBs for Scl+19/Hsp/Venus line with the YFP positive gate, and its percentage of the live cell population, shown in red. (C) Percentage of the YFP+ population (gating shown in B) from day 3–7 for HM-1 (blue), two representative Hsp/Venus control clones (aqua and purple) and two representative Scl+19/Hsp/Venus clones (red and orange), showing the average of three independent differentiation experiments ± standard deviation. (D) Representative flow cytometry plots showing distribution of Scl+19/Hsp/Venus day 4 EB cells in Flk1/CD41 quadrants for all live cells (left plot) and YFP positive cells only (right plot), with the percentage of cells in each quadrant shown in red. (E) Percentage of YFP positive cells in each of the Flk1/CD41 quadrants of (D) for Scl+19/Hsp/Venus (clone 1 in red, clone 2 in orange). Average of three independent differentiation experiments ± standard deviation. (F) As in (D), but for Flk1/CD140a quadrants. (G) As for (E), but for Flk1/CD140a quadrants in (F).