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. 2013 Oct 16;2(11):1270–1278. doi: 10.1242/bio.20135363

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — (A) Left panel: HEK293T was subjected to immunoprecipitation using rabbit polyclonal antibodies against Nup358 (Nup358-IP) or control rabbit IgG (Rb-IgG-IP), and the immunoprecipitates were immunoblotted using indicated antibodies. Right panel: E18 rat brain lysate was subjected to immunoprecipitation using Nup358 antibody. The immunoprecipitate was probed with aPKC (PKCζ) and Nup358 antibodies. (B) The domain structure of PKCζ and the fragments used in the study. (C) Lysates of HEK293T cells expressing HA-tagged fragments of PKCζ were subjected to immunoprecipitation using control Rb-IgG (Rb-IgG-IP) or anti-Nup358 (Nup358-IP) antibodies. The immunoprecipitates were probed with HA-specific antibodies and MAb414 (for Nup358). (D) Cells were co-transfected with HA-PKCζ and GFP-tagged version of BPN, BPM or BPC, and the lysates were subjected to immunoprecipitation using EZview control (Con-IP) or EZview HA (HA-IP) beads (Sigma–Aldrich). Immunoblotting was performed using indicated antibodies.