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. 2013 Oct 16;2(11):1270–1278. doi: 10.1242/bio.20135363

Fig. 5. Nup358 functions upstream of Dvl.

Fig. 5.

(A) Hippocampal neurons were transfected with control siRNA (siControl), Nup358 siRNA (siNup358), Dvl1 siRNA (siDvl1) alone or co-transfected with siNup358 and siDvl1 as indicated. pBetaActin-eGFP (green) was used as the transfection marker. The transfected cells were stained with Tau-1 (red) to study the effect on axon formation. DNA was stained with Hoechst 33342 dye (blue). Scale bar, 25 µm. (B) Quantitative analysis of the effect of siRNA mediated depletion of different proteins on axon formation. Error bars indicate standard deviations, n = 3, **P<0.01, N.S. – non-significant, Student's t test.