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. 2013 Aug 23;2(4):e000361. doi: 10.1161/JAHA.113.000361

Figure 5.

Figure 5.

Specific MEK1/2 inhibitor decreases SERCA2 expression and increases ER stress in Huh‐7 cells in presence of palmitate. (A) A representative Western blot of phosphor‐ERK1/2, SERCA2, phosphor‐IRE1α, BiP, and CHOP in the Huh‐7 cells. (B–F) Quantification of phospho‐ERK1/2/ERK1/2 (B), SERCA2/GAPDH (C), phospho‐IRE1α/IRE1α (D), BiP/GAPDH (E), and CHOP/GAPDH ratio (F) (n=6). Error bars represent SEM. *P<0.05, ***P<0.001 vs. palmitate+DMSO. BiP indicates immunoglobulin heavy‐chain binding protein; BSA, bovine serum albumin; CHOP, C/EBP homologous protein; DMSO, dimethylsulfoxide; ER, endoplasmic reticulum; ERK, extracellular signal‐regulated kinase; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IRE1α, inositol‐requiring enzyme 1α; PD, PD98059; SERCA, sarco/endoplasmic reticulum Ca2+‐ATPase.