Skip to main content
. 2013 Aug 23;2(4):e000096. doi: 10.1161/JAHA.113.000096

Figure 11.

Figure 11.

Effects of silencing Arg‐II in senescent VSMCs on activation of S6K1‐p66Shc and p53 and on PCNA level. Senescent VSMCs were transduced with either rAd/U6‐LacZshRNA or rAd/U6‐Arg‐IIshRNA. Seventy‐two hours after transduction cell lysates were prepared and subjected to (A) immunoblotting analysis of Arg‐II, tubulin, S6K1‐T389 and total S6K1, S6‐S235/236 and total S6, p66Shc‐S36 and total p66Shc, p53‐S15, and total p53. Quantification of the signals is shown in the right bar graphs (n=6). *P<0.05, **P<0.01 vs young cell (Y). B, Immunoblotting analysis and immunofluorescence staining of PCNA. Quantification of the immunoblotting signals of PCNA is shown in the bar graphs underneath the immunoblotting. *P<0.05 vs LacZ‐shRNA. Arg‐II indicates arginase‐II; VSMC, vascular smooth muscle cell; PCNA, proliferating cell nuclear antigen; DAPI, 4′6‐diamidino‐2‐phenyl‐indole, dihydrochloride.