Figure 13.

Enhanced phosphorylation of p66Shc‐S36 accounts for the phenotypic changes of senescent VSMCs. Senescent VSMCs were transduced with either rAd/CMV empty vector as control (con), rAd/CMV‐p66Shc or ‐p66Shc‐S36A (S36A) as indicated. Seventy‐two hours posttransduction, the cells were subjected to (A) H₂DCF staining for detection of H₂O₂. B, JC‐1 staining for analysis of Δψm. C, SA‐β‐gal staining for senescent cells. D, Annexin‐V‐FLUOS staining for apoptotic cells. Quantification of the signals is shown in the corresponding bar graphs (n=6). *P<0.05, **P<0.01 vs con. Scale bar=0.2 mm. Arg‐II indicates arginase‐II; VSMC, vascular smooth muscle cell; H₂DCF, 2′,7′‐dichlorofluorescein; SA‐β‐gal, senescence‐associated β‐galactosidase; rAd, recombinant adenovirus.