Figure 15.

Ablation of Arg‐II in atherosclerosis‐prone ApoE^−/− mice reduces signaling of S6K1, p66Shc, and p53 in the aortas. ApoE^−/−Arg‐II^+/+ and ApoE^−/−Arg‐II^−/− mice were fed a high‐fat diet for 10 weeks. Aortas from mice of both genotypes were cleaned of perivascular tissues and subjected to immunoblotting analysis of Arg‐II, S6K1, p66Shc‐S36 and total p66Shc, p53‐S15 and total p53, and S6‐S235/236 and total S6. Tubulin served as a loading control. Quantification of the signals is shown in the corresponding lower panels (n=4). *P<0.05, **P<0.001 vs ApoE^−/−Arg‐II^+/+. Arg‐II indicates arginase‐II; ApoE, apolipoprotein E; S6K1, ribosomal protein S6 kinase 1.