Lectin‐binding patterns across endothelial cells from different vascular beds. Untreated endothelial cells were surface‐labeled with FITC‐conjugated ConA (A), LCA (B), PHA‐L (C), or SNA (D) as described in the Methods section. A, $P<0.05 vs CoAEC and #P<0.05 vs CtAEC. B, *P<0.05 vs BCECs and #P<0.05 vs CtAECs. Data are mean±SEM All statistical analysis by a single 1‐way ANOVA with Tukey posttest (n=4 to 6 for each condition). BCECs indicates brachiocephalic artery endothelial cells; CtAECs, carotid artery endothelial cells; CoAEC, coronary artery endothelial cells; HAECs, human aortic endothelial cells; HUVECs, human umbilical vein endothelial cells; PmvECs, pulmonary microvascular endothelial cells; SCECs, subclavian artery endothelial cells; ANOVA, analysis of variance; SEM, standard error of the mean; FITC, fluorescein isothiocyanate; ConA, concanavalin A; LCA, lens culinaris agglutinin; PHA‐L, phaseolus vulgaris lectin; SNA, sambucus nigra lectin.