Fig. 3.

Variable (V)-region peptide heat map of compiled de-novo sequencing data from seven patients with primary Sjögren's syndrome (SS) showing public (shared) mutations. (a) Heavy chain V-region sequences align with germline HV3-43 and HV3-30 (IMGT database). (b) Light chain V-region sequences align with germline KV3-20 and KV3-15. (c) Light chain J-regions align with KJ2 and KJ4 germline sequence and heavy chain J-regions aligned with HJ2. Due to a lack of overlapping peptides from the CDR3 region into the J-regions, the exact pairing of V- and J- regions could not be determined. Common mutations divergent from the germline sequence are depicted in the text and colour-coded according to the frequency of the mutation detected in the primary SS patient cohort analysed. Dots indicate amino acids matching to the germline sequence. Complementary determining regions (CDR) regions are boxed.