Fig. 6.

Hepatitis C virus (HCV) infection increases expression of mannan binding lectin-associated serine protease (MASP-1) in the Huh 7·5 hepatocyte cell line. Reverse transcription–quantitative polymerase chain reaction (RT–qPCR) was conducted in the Huh 7·5 hepatocyte cell line 72 h after transfection with either infectious molecular clone JFH-1 or replication defective mutant GND. Mock transfected cells were used as controls. Using the one-way analysis of variance (anova) test with Bonferroni's multiple comparison test to compare MASP-1 expression in mock transfected, Huh 7·5 and GND found that MASP-1 was expressed significantly more highly in Huh7·5 (P = 0·0015).