Fig. 5.

Ang II-induced cardiac up-regulation of MT protein and cell death. Animal treatments and tissue collections are same as those described in Fig. 4. Cardiac MT contents were examined by Western blotting assay (A). Cardiac apoptotic cell death was examined by Western blotting analysis for the cleaved caspase-3 (B) and caspase-12 (C) and CHOP expression (D). Again in order to see whether there was MT induction in the hearts of MT-TG diabetic mice, the exposure time for the films to gels was significantly shorter for samples from MT-TG mice than those from WT mice; Therefore, MT expressions in MT-TG samples looks significantly low than diabetes-induced cardiac MT levels. Experimental data were presented as means ± S.D. (n= 5 at least for each group). *, P< 0.05 versus control.