Table 2.

Primary and secondary antibodies used for IF

Primary antibodies	Catalogue number	Host	Supplier	Dilution
Anti-c-Kit (C-14)	sc-1493	goat	Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA	1:1000
Anti-c-Kit/CD117	A4502	rabbit	DAKO, Carpinteria, CA, USA	1:500
Anti-Spry4	pab0230	rabbit	Covalab, Villeurbanne, France	1:3000
Anti-Spry2	#07—524	rabbit	Upstate, Lake Placid, N Y, USA	1:1000
Anti-Tpbg/5T4	ab45520	rabbit	Abcam, Cambridge, UK	1:200
Anti-Pkrcq/Pkcθ	#2059	rabbit	Cell Signaling Technology, Inc., Danvers, MA, USA	1:100
Anti-Pde3a	[10]	sheep	DSTT Unit, University of Dundee, Scotland	1:3000
Anti-Gja1/Cx 43	#3512	rabbit	Cell Signaling Technology, Inc.	1:500

Secondary antibodies	Catalogue number	Host	Supplier	Dilution
Anti-goat Alexa 448	A11055	donkey	Invitrogen	1.200
Anti-rabbit Alexa 448	A21206	donkey	Invitrogen	1:400
Anti-rabbit biotinylated	711–065-152	donkey	Jackson Immunoresearch, Cambridge, UK	1:200
Anti-sheep biotinylated	713–065-147	donkey	Jackson Immunoresearch	1:200