Figure 1.

Alignment of trypanosomatid mucolipin orthologues with human MCOLN1. The putative transmembrane regions (yellow), PI(3,5)P₂ binding (green) or serine lipase active site (cyan) (LaPlante et al., ²⁰¹¹) are all highlighted. The boxed region represents the cation channel domain. The white triangle corresponds to a V→L alteration seen in MLIV patient 2 (Altarescu et al., ²⁰⁰²), while the black triangle relates to D→Y change that abolishes Fe²⁺ transport in mammalian cells (Dong et al., ²⁰⁰⁸). A transgene carrying the D→K mutation cannot complement MLIV cells (Pryor et al., ²⁰⁰⁶). Identifiers: T.b. – Trypanosoma brucei Tb927.7.950, T.c. –Trypanosoma cruzi TcCLB.508215.6, L.m. – Leishmania major LmjF.26.0990, H.s. – Homo sapiens NP_065394.1 (kinetoplastid genes identified with TriTrypDb/GeneDb codes, mammalian proteins with NCBI locus numbers). Asterisks indicate conserved residues. Alignment was carried out using clustalw2 at http://www.ebi.ac.uk/Tools/msa/clustalw2/.