Figure 2.

Effects of aldosterone (100 nmol/L) on 3-NT protein levels in HUAECs incubated for 24 h in the absence or presence of spiro (1µmol/L). Aldosterone produced an elevation in 3-NT protein level and spiro (1µmol/L) inhibited. Quantitative analysis of 3-NT protein expression, normalized with B-actin, by scanning densitometry. Values for each bar are mean ± SEM from 4 separate experiments and expressed as % of control, p < 0.05 of aldosterones vs control and spiro versus aldosterone. Spiro: Spironolactone; 3-NT: 3-nitrotyrosine