Fig 2.

FRET microscopy reveals interaction between IKK2 and JAB1/Csn5 in the cytosol. 293 cells were transfected with CFP and YFP separately (neg. control, CFP / YFP), a fusion protein of CFP and YFP (pos. control, CFP-YFP fusion), CFP-IKK2 in combination with either YFP-tagged full-length JAB1 (CFP-IKK2, YFP-JAB1) or YFP-tagged JAB1 N-terminal domain comprising the first 166 amino acids (CFP-IKK2, YFP-JAB1NT). Images were acquired with CFP-, YFP and raw-FRET filter sets and a pseudo-coloured corrected FRET image (FRETc) was calculated using ImageJ with the plug-in PixFRET [41].