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. 2009 Jul 28;14(6b):1555–1568. doi: 10.1111/j.1582-4934.2009.00866.x

Fig 3.

Fig 3

Interaction of endogenous JAB1/Csn5 with IKK2 in 293 cells. (A) Immunoprecipitation of the IKK-complex was carried out with anti-IKK2 antibodies (IKK) or an unrelated control antibody (con) as indicated in the header followed by immunoblot detection of IKK2 (upper panel) and JAB1 (lower panel) in immunoprecipitates (IP) and extracts as indicated. (B) Immunoprecipitation of the endogenous CSN complex with anti-Csn7 antibodies (Csn7) or unrelated control antibodies (con) followed by immunoblot detection of IKK2 (upper panel) and JAB1/Csn5 (lower panel). (C) Interaction of endogenous IKK-complexes with SCF-complexes. Cell extracts were subject to immunoprecipitation (IP) with anti-IKK2 and anti-NEMO antibodies (+) or unrelated control antibodies (–) followed by immunoblotting with anti-IKK2, Cullin-1 and βTrCP antibodies as indicated.