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Effect of JAB1/Csn5 on apoptosis. (A) Stable JAB1 knock-down cells (siJAB1) are protected from apoptosis induced by serum-withdrawal (24 h) or TNFα (50 ng/ml). The percentage of apoptotic cells was determined by flow cytometry staining with FITC-Annexin V. Data are mean values of triplicates; error bars reflect SD. (B) Apoptosis assay as in (A) after ectopic expression of the constructs indicated.
