Fig 9.

Podosome-like structures, invadopodia and invadopodial cell extensions are resistant to inhibitors of actin and tubulin polymerization. The dynamics of EGFP-actin were monitored using wide-field time-lapse microscopy for 30 min. after applying the inhibitors. Treatment with cytochalasin B (10 μM), an inhibitor of actin polymerization, did not change the morphology of podosomes or invadopodia, although cell shape and cytoskeletal actin organization underwent major alterations. The club-ended cell extensions could still be seen in 43B cells after disruption of the actin cytoskeleton (arrows). Scale bar: 20 μm.