Table 1.

Gene constructs, antibodies and antisera used^*

Target	Antibody	Reference
EGFP-actin		BD Biosciences, San Jose, CA, USA
EGFP-cortactin		[22]
EGFP-filamin A		[23]
Arp 2/3	Pc rabbit antiserum	Upstate/Millipore, Charlottesville, VA
Cortactin	4F11	Upstate/Millipore
F-actin	rhodamine phalloidin	Molecular Probes/Invitrogen, Eugene, OR
Filamin A	PM6/317	Chemicon/Millipore, Temecula, CA, USA
FAK	2A7	Upstate/Millipore
Integrin α3 subunit	J143	[24]
Integrin αv subunit	LM142.69	[25]
Integrin β1 subunit	102DF5	[26]
Integrin β5 subunit	1A9	[27]
MT1-MMP	LEM-2/15	[28]
Nucleus	TO-PRO-3	Molecular Probes/Invitrogen
C-terminal region of human pacsin 2	Pc rabbit antiserum	Abgent, San Diego, CA
Phosphotyrosine	PY20	Molecular Probes/Invitrogen
Plectin	HD-121	[29]
αII-spectrin	101AA6	[30]
Talin	MCA725S	AbD Serotec/MorphoSys, Oxford, UK
Tensin	Clone 5	BD Biosciences
β-tubulin	DM3B3	[31]
Vinculin	Pc rabbit antiserum	[4]

^*Antibodies are monoclonal unless otherwise stated. Pc: polyclonal.