Fig 5.

Effect of replication inhibition on ssODN-mediated gene targeting. (A) Msh2-deficient ES cells carrying the mutant neo gene were incubated with increasing concentrations of aphidicolin or hydroxyurea (C) between 6 hrs before and 22 hrs after exposure to ssODN 3N. Relative targeting efficiencies are the number of G418-resistant colonies per 10⁵ cells that were plated after ssODN exposure, normalized to the efficiency found in untreated control cells (black bars). Corrected targeting efficiencies are adjusted for the decreased colony survival after treatment with aphidicolin or hydroxyurea (white bars). Error bars represent the S.D. of three independent experiments. (B) Colony-forming ability of Msh2-deficient ES cells after exposure to increasing doses of aphidicolin or hydroxyurea (D) between 6 hrs before and 22 hrs after ssODN exposure. Error bars represent the S.D. of three independent experiments.