Fig 6.

Interaction between the αBE4 binding proteins is induced in MHC-CnA mice. (A) Cardiac nuclear protein extracts from WT and MHC-CnA (CN) mice were subjected to SDS-PAGE fractionation followed by western blot with anti-phospho-Stat3 (Tyr705) antibody (that detects only Stat3 phosphorylated at tyrosine 705), anti-NFATc3, anti-NFATc4 and anti-Nished antibodies (n= 2). Lamin B was used as a loading control. Quantification of protein levels in the (WT) and MHC-CnA (CN) mice. (B) Cardiac cell lysates from WT and MHC-CnA (CN) mice were immunoprecipitated (IP) with anti-STAT3 antibody and western blotted (WB) with anti-NFATc4, anti-NFATc3 and anti-STAT3 antibodies. Data shown represent one of two separate experiments, with two individual mice per experiment.