Fig 8.

Immunocytochemical localization of STAT3 and NFATc3 in En-1 induced and AG490 treated rat primary cardiomyocytes. Cultured neonatal primary cardiomyocytes were treated with vehicle (DMSO) (left panel), En-1 (50 nM) (middle panel), or En-1 (50 nM) with AG490 (5 μM) (right panel), then immunostained for sub-cellular localization of STAT3 and NFATc3. Nuclei were stained with 4′, 6-diamidino-2-phenylindole (Dapi) and merged patterns are shown in the bottom panel. All images were obtained at 40× magnification.