Fig 1.

Prevention of the norepinephrine-induced increases in PLC isozyme mRNA levels by siRNA transfection of cardiomyocytes. Representative blots (A) showing PLC isozyme β₁ (114bp), β₃ (230bp), γ₁ (123bp) and δ;₁ (190bp) mRNA levels relative to GAPDH (138bp) mRNA level in LV cardiomyocytes. Quantified data shows PLC β₁ (B), β₃ (C), γ₁ (D) and δ;₁ (E) mRNA levels relative to GAPDH mRNA level in cardiomyocytes transfected with 5 nM PLC β₁, β₃, γ₁ and γ₁ siRNA and treated with NE (5 μM) for 2 hrs. Control cardiomyocytes were transfected with 5 nM negative control siRNA as described in ‘materials and methods’ section. Values are mean ± S.E. of five experiments performed with five different cardiomyocyte preparations. *Significantly different (P < 0.05) versus control; ^#significantly different (P < 0.05) versus NE. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; CONT, control; NC, negative control; NE, norepinephrine; siRNA, small interfering RNA.