Fig 3.

Inhibition of norepinephrine-induced increases in c-Fos and c-Jun mRNA levels in cardiomyocytes transfected with PLC isozymes siRNA. Representative blots (A) and quantified data (B) and (C) showing c-Fos (74 bp) and c-Jun (163 bp) mRNA levels respectively relative to GAPDH (138 bp) mRNA level in LV cardiomyocytes transfected with 5 nM PLC isozyme β₁, β₃, γ₁ and δ;₁ siRNA and treated with NE (5 μM) for 2 hrs. Control cardiomyocytes were transfected with 5 nM negative control siRNA as described in ‘Materials and methods’ section. Values are mean ± S.E. of five experiments performed with five different cardiomyocyte preparations. *Significantly different (P < 0.05) versus control; ^#significantly different (P < 0.05) versus NE. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; CONT, control; NC, negative control; NE, norepinephrine; siRNA, small interfering RNA.